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  • Drag-and-drop it directly onto the ImageJ toolbar.
  • File > Open


If your image doesn't open correctly

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To make sure Bio-Formats is used, Edit > Options > ImageJ2 and uncheck Use SCIFIO when opening files.

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Not only does it read the image data, it also understands the scale information and lets you view details about the image acquisition. That means you do not have to convert your files into TIFF to use them in ImageJ -- in fact, it's better to keep them in the original format.


To deal with the many possible kinds of images, Bio-Formats displays Import Options when you open a file.

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